112 FlowJo Keyboard Shortcuts

Photo of Pratik PratikLast Updated: November 11, 2025
165 3 minutes read

Learn FlowJo Shortcuts for Windows

Workspace Shortcuts

ActionFlowJo Shortcuts
Close WorkspaceCtrl + W
ExitCtrl + Q
UndoCtrl + Z
Customize RibbonsCtrl + ]
New WorkspaceCtrl + N
Add SamplesCtrl + ;
New GroupCtrl + G
Open Layout EditorCtrl + L
Open Table EditorCtrl + T
PreferencesCtrl + Y
Panel WizardCtrl + Shift + P
Open WorkspaceCtrl + Num/
PrintCtrl + P
SaveCtrl + S
Export / ConcatenateCtrl + E
Save AsCtrl + Shift + S
FindCtrl + F
FCS ScanCtrl + Shift + F
CutCtrl + X
PasteCtrl + V
ClearCtrl + Delete
Select AllCtrl + A
Select Equivalent NodesCtrl + Shift + E
Collapse AllCtrl + Shift + U
Expand AllCtrl + U
Create GroupCtrl + G
Copy Gate to GroupCtrl + Shift + G
Open Graph WindowCtrl + Enter
InspectCtrl + I
RenameCtrl + R
Add StatisticCtrl + B
Add KeywordCtrl + Shift + I
Create Group from Keyword ValueCtrl + J
Create Keyword Value SeriesCtrl + 6
CompensationCtrl + M
Matrix EditorCtrl + Shift + M
Check Samples QualityCtrl + Shift + V
Derived ParametersCtrl + Shift + D
Create Combination GatesCtrl + ,
Make Or GateCtrl + Shift + O
Make And GateCtrl + Shift + A
Make Not GateCtrl + Shift + N
Make Identical GatesCtrl + =
Plate EditorCtrl + 9
Show in 3DCtrl + 3
Edit ColumnsCtrl + Shift + K
Hide/Show StatisticsCtrl + Shift + B
Show/Hide Comp NodesCtrl + Shift + C

Layout Editor Shortcuts

ActionFlowJo Shortcut Keys
Add A New LayoutCtrl + N
Duplicate LayoutCtrl + =
Delete LayoutCtrl + –
PrintCtrl + P
Save Image AsCtrl + S
View WorkspaceCtrl + 0
Open Original GraphCtrl + O
PropertiesCtrl + I
UndoCtrl + Z
CutCtrl + X
PasteCtrl + V
Select AllCtrl + A
Figure Presets (Apply)Ctrl + +
Bring ForwardCtrl + F
Bring to FrontCtrl + Shift + F
Send BackwardCtrl + B
Send to BackCtrl + Shift + B
GroupCtrl + G
UngroupCtrl + U
TopsCtrl + T
BottomsCtrl + \
Vertical CenterCtrl + Page Down Key
Vertical SpacingCtrl + K
LeftCtrl + L
RightCtrl + R
Horizontal CenterCtrl + H
Horizontal SpacingCtrl + J

Table Editor Shortcuts

ActionFlowJo Shortcuts
New TableCtrl + N
Duplicate TableCtrl + =
Remove TableCtrl + –
For DisplayCtrl + D
Batch to LayoutCtrl + L
Batch to ClipboardCtrl + K
Batch to PrinterCtrl + P
To FileCtrl + F
Select AllCtrl + A
ClearCtrl + Delete
Add ColumnCtrl + Shift + N
Edit ColumnCtrl + E

Graph Window Shortcuts

ActionFlowJo Shortcuts
Close WindowCtrl + W
Close All Graph WindowsCtrl + Shift + W
Save Graphic asCtrl + S
PrintCtrl + P
UndoCtrl + Z
ClearCtrl + Delete
Copy Image with TransparencyCtrl + I
Copy Histogram FunctionCtrl + F
Copy to Layout EditorCtrl + L
Add StatisticCtrl + B
PreferencesCtrl + ,
Duplicate ViewCtrl + D
Transpose AxesCtrl + T
Manually Enter GateCtrl + G
Make MovieCtrl + M
Show in 3DCtrl + 3
Show WorkspaceCtrl + 0
Up to Parent PopulationCtrl + U
Open Child PopulationCtrl + O
Previous SampleCtrl + Page Down Key
Next SampleCtrl + Page Up Key
Previous XCtrl + J
Next XCtrl + K
Previous YCtrl + F10
Next YCtrl + Y

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Photo of Pratik PratikLast Updated: November 11, 2025
165 3 minutes read
Photo of Pratik

Pratik

Pratik is the founder of Tutorial Tactic and a productivity tools specialist with 15 years of hands-on experience in Google Workspace, Microsoft Office, and software automation. He has published over 1,500 guides on keyboard shortcuts, software commands, how-to tutorials and workflow optimization, helping readers across the US and India work faster with the tools they use every day. Tutorial Tactic was founded in 2021 with one goal: cut through the noise and give readers exactly what they need fast, verified, and beginner-friendly.
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